Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment.

Masahiro Ito,Makoto Nagane

doi:10.1271/bbb.65.2773

Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment.

Masahiro Ito, Makoto Nagane

https://doi.org/10.1271/bbb.65.2773

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Journal: Bioscience, Biotechnology, and Biochemistry	Publication Date: Jan 1, 2001
Citations: 38	License type: free

Affiliation: Toyo University

#High Electric Field Strength #Electro-transformation Method #Glycine Treatment #High Osmolarity #Transformation Efficiency #High Glycine #Optimum Conditions #High Field Strength #Efficiency Improved #High Field

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An electro-transformation method was established for facultatively alkaliphilic Bacillus pseudofirmus OF4. A high osmolarity electroporation medium with a high electric field strength was effective. Transformation efficiency improved 110-fold in glycine-treated cells compared to non-glycine-treated cells. Under optimum conditions, the transformation efficiency was 1.69 x 10(6) transformants per microg of pUB110.

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Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment.