Abstract
Glutamate oxidase (LGOX) is a kind of flavin proteases that can specifically catalyze the deamination of L-glutamate to α-ketoglutarate. It can be widely used in factory production, biosensors, clinical biochemical testing, etc. Therefore the directed evolution technology was used to construct a mutation library through error-prone PCR, and four mutants with increased enzyme activity were obtained through high-throughput screening: F94L, S280T, I282M, H533R. The S280TH533L mutant with the highest enzyme activity was obtained, and its enzyme activity increased by 90.7% compared with the wild type.
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