Abstract
A possible source of biological material for the production of biodiesel is represented by microalgae, in particular by their lipid content. The aim of the present work was to optimize culture medium composition for improving growth and lipid content of green microalgae Chlorella sorokiniana, Scenedesmus acuminatus and blue green Cyanobacterium aponicum. Lipids were quantitatively determined by spectrofluorometric method using Nile red flurometric stain. Initially, the effect of two different medium types, Bold’s and optimized culture medium (OCM), four types of carbon source (glucose and sodium acetate, molasses, glycerol, control) and four nitrogen concentrations (100%, −75%, −50%, −25%) on the enhancement of biomass and lipid content and lipid productivity were studied; indeed, optimized culture medium significantly improved growth, CDW for three microalgae, beside increasing lipid content and lipid productivity for S. acuminatus and C. aponicum by 7.5 and 5 folds respectively at 25 th day compared to Bold’s medium. Moreover, 25% nitrogen deficient medium significantly in
Highlights
Microalgae display high areal productivity, and some of them are able to accumulate significant amounts of lipids
The highest CDW was obtained on the 20th day of incubation for C. aponicum and C. sorokiniana, increasing 2.2 and 1.8 folds respectively, over Bold’s medium while the highest CDW for S. acuminatus was obtained on the 25th day by 1.5 folds increase compared to Bold’s medium at same day
With respect to lipid productivity generally, the highest values of lipid productivities was achieved at exponential phases for three tested microalgae; C. sorokiniana recorded the highest lipid productivity 13.8 mg·g−1·d−1 followed by S. acuminatus 9.1 mg·g−1·d−1 under Bold’s medium they began to decline to its lowest values on the 25th day
Summary
Microalgae display high areal productivity, and some of them are able to accumulate significant amounts of lipids. They are seen as promising candidates for the industrial production of biodiesel [1]. To expand this novel feedstock, research and development is needed in several domains, from the selection of suitable strains to the optimization of the different steps required for mass scale operation (biomass production, harvesting, lipid extraction) [1]. Fast growth promotes high biomass productivity which increases yield per harvest volume in a certain period (productivity) and decreases cost.
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