Abstract
In fermentation technology, strain improvement of baker's yeast has traditionally relied on random mutagenesis followed by screening for mutant exhibiting enhanced properties of interest. Such mutant organisms are useful in several industries. Saccharomyces cerevisiae can use sucrose as the sole source of both carbon and energy; hydrolysis of this sugar is catalyzed by the enzyme invertase. The main objective of this work is to overcome the glucose repression of invertase by invertase constitutive mutants through UV mutation. This may occur in any glucose repressible genes as a single or double mutation in repressor gene (s) which might cause constitutive synthesis of invertase. These mutated screened strains were optimized with various glucose concentration and different incubation hours for higher invertase production. The maximum synthesis of invertase was 1.066 units/ml in hour from mutant Saccharomyces cerevisiae type-2 strain.
Highlights
Saccharomyces cerevisiae is the most thoroughly investigated eukaryotic microorganism, which aids our understanding the biology of the eukaryotic cell
S. cerevisiae growing under repressible condition (1% glucose or more) could produce a burst of external invertase when shifted to higher temperature
These results are consistent with the hypothesis that invertase is continuously synthesized both in the presence and absence of glucose, but it is degraded under repressible condition [14]
Summary
Saccharomyces cerevisiae is the most thoroughly investigated eukaryotic microorganism, which aids our understanding the biology of the eukaryotic cell. S. cerevisiae growing under repressible condition (1% glucose or more) could produce a burst of external invertase when shifted to higher temperature. The secretion of this invertase requires protein synthesis, but it was found to be independent of RNA formation. The level of accumulated and translated mRNA was inversely proportional to the glucose present in the growth medium [13] These results are consistent with the hypothesis that invertase is continuously synthesized both in the presence and absence of glucose, but it is degraded under repressible condition [14]. Mutation occurs in one of the suppressor genes, snf, causes constitutive synthesis of invertase and other repressible enzymes [17].
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