Improvement Of Inactivated Equine Herpes Virus-1 Vaccine Using Carbomer

Safaa A Warda,Ekbal Farouk,Lubna F Farahat,Naglaa I Ali,Fatma F Warda

doi:10.21608/javs.2021.140080

Abstract

An inactivated Equine herpes virus-1 vaccine was successfully improved using carbomer as adjuvant inducing high and long immunity in vaccinated mares in comparison with the convention one adjuvanted with Al-hydra gel and saponin. Such purpose was established by using 0.5% carbomer as adjuvant to the inactivated EHV-1. The applied quality control tests carried out on such vaccine revealed that it is free from foreign contaminants, safe in pregnant mares and mice and potent induced high levels of specific EHV-1 antibodies in vaccinated Guinea pigs and mares as measure by ELISA and SNT. This immunity was sufficient to protect vaccinated horses up to 28 weeks (7months) post-vaccination.

Highlights

Contaminants, safe in pregnant mares and mice and potent induced high levels of specific Equine herpes virus-1 (EHV-1) antibodies in vaccinated Guinea pigs and mares
No virucidal nature and efficacy in one shot vaccination schedules; a lightly cross-linked polymer of acrylic has become widely used as an adjuvant in the veterinary field (Diamantstein et al, 1971) enhance the strength and duration of antibody responses stimulated by inactivated equine influenza vaccines as compared with vaccines of equivalent antigenic content (Wood et al, 1983)
Equine herpes virus-1 (EHV-1) Locally isolated EHV-1 isolated by Hassanein et al (2002) and adapted on Vero cell culture by Safaa (2007) identified by reference freeze-dried rabbit antiEHV-1 was supplied by the Department of Equine Vaccine Research (DEVR); Veterinary Serum and Vaccine Research Institute (VSVRI) and used for an inactivated vaccine

Summary

Introduction

Contaminants, safe in pregnant mares and mice and potent induced high levels of specific EHV-1 antibodies in vaccinated Guinea pigs and mares. Equine herpesvirus type 1 (EAV-1) causes abroad range of manifestations in horses, including a central nervous system disease involving the spinal cord and brain (myeloid cephalopathy), respiratory disease, abortions and perinatal death (Meaquita et al, 2017). In recent years, increased incidence of Equine herpesvirus myeloid Cephalopathy (EHM) has been observed by infection with the same virulent strains of EHHV-1 leading to inflammation of the blood vessels supply the brain and spinal cord (Henninger et al, 2007). No virucidal nature and efficacy in one shot vaccination schedules; a lightly cross-linked polymer of acrylic has become widely used as an adjuvant in the veterinary field (Diamantstein et al, 1971) enhance the strength and duration of antibody responses stimulated by inactivated equine influenza vaccines as compared with vaccines of equivalent antigenic content (Wood et al, 1983).

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Conclusion