Abstract
Cyanide usage in gold processing techniques has become increasingly challenging due to its toxicity and environmental impact. It is possible to develop environmentally friendly technology using thiosulfate because of its nontoxic characteristics. Thiosulfate production requires high temperatures, resulting in high greenhouse gas emissions and energy consumption. The biogenesized thiosulfate is an unstable intermediate product of Acidithiobacillus thiooxidans sulfur oxidation pathway to sulfate. A novel eco-friendly method was presented in this study to treat spent printed circuit boards (STPCBs) using biogenesized thiosulfate (Bio-Thio) obtained from Acidithiobacillus thiooxidans cultured medium. To obtain a preferable concentration of thiosulfate among other metabolites by limiting thiosulfate oxidation, optimal concentrations of inhibitor (NaN3: 3.25 mg/L) and pH adjustments (pH= 6–7) were found to be effective. Selection of the optimal conditions has led to the highest bio-production of thiosulfate (500 mg/L). The impact of STPCBs content, ammonia, ethylenediaminetetraacetic acid (EDTA), and leaching time on Cu bio-dissolution and gold bio-extraction were investigated using enriched-thiosulfate spent medium. The suitable conditions were a pulp density of 5 g/L, an ammonia concentration of 1 M, and a leaching time of 36 h, which led to the highest selective extraction of gold (65 ± 0.78%).
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