Abstract
Curcumin (Cur) has many potential applications in the food industry. However, it suffers from poor water solubility, low plasma and tissue uptake and susceptibility to oxidation. In this study, β-lactoglobulin (β-LG)-hyaluronic acid (HA) binary covalent complexes were prepared by ultrasound-assisted Maillard reaction and used as the carrier to deliver Cur. Results of SDS-PAGE, degree of grafting, and SEM showed that β-LG was successfully grafted to HA, and the degree of grafting increased from 15.30 ± 0.28 % to 29.70 ± 0.45 % under ultrasound treatment. There was a decrease in α-helix and β-sheet contents, and an increase in random coil content, and the amide I bands, and tryptophan motifs in the covalent secondary and tertiary structures of the nanocomplexes. Encapsulation by β-LG-HA could form the controlled release of Cur, resulting in a decreased release rate in the oral and gastric phases and an increased release rate in the intestinal phase, as demonstrated in an in vitro digestion simulation. Furthermore, the β-LG-HA-Cur nanocomplex exhibited the superior antioxidant and enzyme inhibition of α-amylase and α-glucosidase. The inhibition rates of α-amylase and α-glucosidase could reach 48.91 ± 0.81 % and 49.88 ± 0.61 %, respectively. This study will help to understand the ultrasound-assisted Maillard reaction covalent binding of protein- polysaccharides complexes, demonstrate the potential for Cur delivery, and develop functional dairy products with hypoglycemic activity.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call