Improvement in Menopause-Associated Hepatic Lipid Metabolic Disorders by Herbal Formula HPC03 on Ovariectomized Rats

Boyoon Chang,Sungyeon Kim,Dae Sung Kim

doi:10.1155/2020/1409376

Abstract

Postmenopausal women have an increased risk of developing nonalcoholic fatty liver disease (NAFLD). We formulated a combination of three herb mixtures (HPC03) and observed lipid-lowering efficacy. HepG2 cells were treated with oleic acid to induce an NAFLD model (in vitro). Also, we investigated potential of HPC03 in an ovariectomize- (OVX-) induced NAFLD model (in vivo). We separated the mice into six groups, as follows: SHAM, OVX, OVX + β-estradiol, and OVX + HPC03 (50, 100, and 200 mg/kg). Rats were administered with/without HPC03 for 12 weeks. HPC03 dose dependently inhibited the lipid accumulation involved in lipogenesis in HepG2 cells. The body weight, fat mass, and weights of the liver were decreased in the OVX group than that in the other groups. HPC03 had decreased adiposity that was induced by OVX. HPC03 treatment reduced liver lipid deposition and prevented the increase in serum and liver triglyceride export when there was a deficiency in estradiol. HPC03 improves OVX-induced fatty liver and lipid metabolism. These findings suggest that HPC03 from postmenopausal women has a protective effect during NAFLD conditions.

Highlights

Nonalcoholic fatty liver disease (NAFLD) is a broad concept that includes an excessive accumulation of triglyceride in the liver, from nonalcoholic steatohepatitis and cirrhosis [1,2,3]
We previously reported that the herbal formula HPC03 increased the cell growth and the estrogen-dependent genes, such as Psen2, Pgr, and Ctsd, and stimulated proliferation of ER-positive MCF-7 cells
We investigated the nonalcoholic fatty liver disease (NAFLD) protection of HPC03, which exhibits estrogen-like action

Summary

Introduction

Nonalcoholic fatty liver disease (NAFLD) is a broad concept that includes an excessive accumulation of triglyceride in the liver, from nonalcoholic steatohepatitis and cirrhosis [1,2,3]. In the earliest stages of nonalcoholic fatty liver disease, the triglyceride content among the healthy and dry people with simple lipidosis falls into the top 95% or more and intracellular defined when the proportion of neutral fat particles is 5% or more [4]. E prevalence of nonalcoholic fatty liver was reported to increase in men in their 30 s–40 s and decreased, while women tended to increase sharply in their 60 s after menopause. Menopausal women experience significant changes in hormones due to a decrease in the female hormone estrogen. Estrogen is decreased cholesterol is accumulated in the body, and fatty liver is induced. Due to the effects of these hormones, women are more likely to be exposed to fatty liver than men [9, 10]

Results

Discussion

Conclusion