Abstract
BackgroundThe increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has followed the international rise in obesity rates. Multiple mechanisms are involved in NAFLD, including endoplasmic reticulum stress and oxidative stress. Heat shock protein 70 (HSP70), which is abundant in most organisms, is sensitive to stress. However, the role of HSP70 in NAFLD has not been investigated. Here, we investigated the possible role of HSP70 in lipid synthesis.MethodsC57BL/6 mice were fed a high-fat diet, and HepG2 cells were treated with 0.5 mM palmitic acid (PA). HSP70 expression was detected by qPCR, Western blot and immunohistochemistry. Total cholesterol (TC) and triglyceride (TG) levels were detected by enzyme-linked immunosorbent assay (ELISA). After Hsp70 overexpression and knockdown, TC and TG levels and FAS, SCD, and ACC expression were detected.ResultsHSP70 expression was significantly increased in the livers of obese mice. In vitro, HSP70 expression was markedly induced by PA in HepG2 cells. Notably, HSP70 overexpression in HepG2 cells enhanced TC and TG synthesis, in parallel with the upregulation of lipogenic genes, including FAS, SCD and ACC. By contrast, HSP70 knockdown decreased the levels of cellular lipids and the expression of FAS, SCD, and ACC in HepG2 cells. Together, our results suggest that HSP70 may promote lipogenesis in HepG2 cells.ConclusionsHeat shock protein 70 promotes lipogenesis in HepG2 cells.
Highlights
The increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has followed the international rise in obesity rates
The specific role of Heat shock protein 70 (HSP70) in NAFLD remains unclear. In view of these considerations, we studied the changes in HSP70 expression in mice with NAFLD and the effect of HSP70 on hepatocyte lipogenesis
HSP70 expression is up-regulated in fatty liver To examine the association between HSP70 and fatty liver, we first evaluated the expression of HSP70 in the livers of obese mice
Summary
The increasing prevalence of non-alcoholic fatty liver disease (NAFLD) has followed the international rise in obesity rates. Multiple mechanisms are involved in NAFLD, including endoplasmic reticulum stress and oxidative stress. Heat shock protein 70 (HSP70), which is abundant in most organisms, is sensitive to stress. The role of HSP70 in NAFLD has not been investigated. We investigated the possible role of HSP70 in lipid synthesis. Multiple mechanisms are involved in the development of NAFLD, including endoplasmic reticulum (ER) stress and oxidative stress. Heat shock protein 70 is the most well-studied in response to stress and has been identified as a significant modulator of adaptation during thermal stress in domestic animals [2]. The role of HSP70 in stress reactions varies, and research findings have been inconsistent.
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