Abstract
We investigated the efficiency of the 5� -untranslated region (UTR) of the tobacco alcohol dehydrogenase gene (NtADH-5� UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, ADH� , cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::b-glucuronidase (GUS); NtADH-5� UTR with a spacer, ADHS, CaMV 35S promoter::NtADH-5� UTR::25-bp spacer::GUS; and NtADH-5� UTR with no spacer, ADH� , CaMV 35S promoter::NtADH-5� UTR::GUS. The highest GUS activity in ADHS and ADHfor chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADHplants. NtADH-5� UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH� ), 47 to 568 (ADHS), and 267 to 1360 (ADH� ) for chrysanthemum, and 1 to 3 (ADH� ), 47 to 114 (ADHS), and 85 to 226 (ADH� ) for torenia. NtADH-5� UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.
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