Abstract
The spiral disk for type-J high-speed counter-current chromatography (HSCCC) has been improved by placing short segments of PTFE (tetrafluoroethylene) tubing into the channel at regular intervals. The best results were obtained with a four-channel spiral column using 600 spacers, which significantly improved stationary phase retention and partition efficiency in both n-butanol–acetic acid–water (4:1:5) and poly(ethylene glycol) 1000-dibasic phosphate polymer phase systems. Based on these findings, a “bead-chain spiral disk” was designed and its performance tested with a type-J HSCCC centrifuge. This new column design substantially improved the separation of both dipeptide and protein samples in terms of stationary phase retention and partition efficiency.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
