Abstract
We developed a micro method of analyzing chlordane compounds (CLD) in mouse tissues by improving a United States Environmental Protection Agency (U.S. EPA) procedure. The micro method consisted of acetone/hexane extraction, purification by hexane/acetonitrile partition followed by Florisil column chromatography, and detection by capillary GC equipped with an electron capture detector (ECD). CLD isomers were recovered to a level of 94–107% from spiked control tissue samples (liver: 2 or 20 ng of CLD; kidney, spleen, brain, adipose fat: 10 ng of CLD) by means of correcting by 2 ng of aldrin internal standard. This method was applicable to liver samples weighing less than 0.30 g as well as kidney, spleen, brain and adipose tissue. This rapid and inexpensive method, which was also highly sensitive (ppb on a whole wet tissue basis), could be applied to the analysis of other organic chlorinated compounds, such as isomers of BHC and DDT, endrin and dieldrin, with minor changes in the Florisil column chromatography.
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