Abstract
The trimethylsilyl (TMS) derivative of sterigmatocystin showed high response to a electron-capture detector, so the micro analysis of sterigmatocystin in cereals was investigated by gas-liquid chromatography with electron-capture detector (ECD-GLC).The sterigmatocystin was extracted with a mixture of methanol and 1% Sodium chloride solution (55:45) from sample and was re-extracted with chloroform.After cleaned up by silica gel plus Florisil column chromatography and Sephadex LH-20gel permeation chromatography, it was silylated with N, O-bis (trimethylsilyl) trifluoroacetamide in ethyl acetate. The TMS-derivative was determined by ECD-GLC with a column (3mm×50cm) of 2% OV-17 on gas-chrom Q.The limit of detection by the present method was about 10ppb and recoveries of sterigmatocystin added to various cereals at the level of 40ppb were 82.3-92.0%.
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More From: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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