Improved methane production with redox-active/conductive biochar amendment by establishing spatial ecological niche and mediating electron transfer

Abstract

The multifunctional roles of biochar as an additive in improving the performance of anaerobic digestion (AD) has not been perfectly understood. In this study, the effects of different biochars on AD and the enhanced mechanisms were explored. The CH4 productions were significantly improved with an increase of 45.9%, 28.3% and 16.5% by amendment with biochar pyrolyzed at 300℃ (BC300), 450℃ (BC450) and 600℃ (BC600), respectively. The tightly-bound communities were established on biochar at the initial stage of fermentation and functional microbes were selectively enriched/colonized in biochar-amended systems. Distinctive loosely-bound microbial communities were observed in BC300 and BC600 amended systems, among which electroactive Desulforhabdus and Clostridiales were the dominant bacteria. Biochar amendments also led to the formation of distinctive spatial ecological niches and the selection preference of microbes for specific spatial locations. These results provided new insights in revealing the potential mechanisms of enhanced AD performance by biochar amendment.