Improved Metabolism of Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; in Human Osteoblasts Cells After Biofield Energy Healing Treatment

Vaibhav Rajan Parulkar

doi:10.11648/j.ajlm.20180301.13

Abstract

Bone formation, remodeling, mineralization, resorption, and healing processes involves a coordinated action of different cell types, which are very prevalent in the elderly population. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were studied. The cell viability using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 304.6%, 237.2%, and 110.6% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI, while 356.9%, 186.2%, and 87.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+BT-TI group as compared to the untreated group. Collagen content was significantly increased up to 163.7%, 114.4%, and 22.7% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 190.1%, 64.9%, and 50% increased collagen at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 330% and 121.5% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+BT-TI group, while 146.7% and 305.9% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+UT-TI group, as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium, and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Highlights

Vitamin D has multiple effects, which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems
In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500 mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5]
Rutin hydrate was purchased from TCI, Japan, while vitamin D3 and L-ascorbic acid were obtained from Sigma-Aldrich, USA

Summary

Introduction

Vitamin D has multiple effects, which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems. Calcitriol play an important role in maintaining the normal level of calcium and phosphorus, promotes bone mineralization, induce or repress the genes responsible for conserving the mineral homeostasis and skeletal integrity, and inhibit hypertension, kidney damage, cardiovascular and immune disorders (such as Lupus, Addison Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and the secondary hyperparathyroidism [3]. In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500 mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5]

Methods

Results

Conclusion