Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions.

Soo Kweon Lee,Youngsang Chun,Ju Hun Lee,Chulhwan Park,Seung Wook Kim,Hah Young Yoo,Hyeong Ryeol Kim,Ja Hyun Lee

doi:10.3390/biom9090461

Soo Kweon Lee, Youngsang Chun + Show 6 more

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https://doi.org/10.3390/biom9090461

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Abstract

Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.

Highlights

Cordyceps militaris, widely used as a traditional medicinal mushroom due to various biological activities of the body since ancient times in Asia, is a fungus belonging to the family Clavicipitaceae and a species of Cordyceps genus [1]. in general, Cordyceps has unique behaviors such as winter-insect and summer-plant form
The cultural broth was centrifuged at 8000× g for 30 min at 4 ◦ C, and the sediment was washed by distilled water. the dry cell weight (DCW) was measured by the weight of samples through a pre-weighed filter paper (Whatman GF/C, Maidstone, UK) and dried in a vacuum oven (VO-20X, Jeiotech, Daejeon, Korea) for 48 h at 50 ◦ C
The highest cell growth of about 290 mg/L was by 150 rpm at 4 days. These results indicated that shaking speed of 150 rpm was the most effective one achieved by 150 rpm at 4 days. These results indicated that shaking speed of 150 rpm was the most for both cordycepin production and cell growth compared to other speeds

Summary

Introduction

Widely used as a traditional medicinal mushroom due to various biological activities of the body since ancient times in Asia, is a fungus belonging to the family Clavicipitaceae and a species of Cordyceps genus [1]. in general, Cordyceps has unique behaviors such as winter-insect and summer-plant form. In general, Cordyceps has unique behaviors such as winter-insect and summer-plant form. Widely used as a traditional medicinal mushroom due to various biological activities of the body since ancient times in Asia, is a fungus belonging to the family Clavicipitaceae and a species of Cordyceps genus [1]. Their spores can enter into a specific living insect and kill the host by feeding. Their hyphae can grow from inside of the host. Cordycepin (30 -deoxyadenosine), one of nucleoside analogues, was first isolated from the medicinal mushroom Cordyceps militaris. the difference between cordycepin and adenosine is the lack of the 30 -hydroxyl

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