Abstract
Plant biotechnology (micropropagation, secondary metabolites, etc.) is the science of growing different plant cells under controlled artificial environment conditions. Thence, successful plant biotechnology depends on the techniques of plant tissue culture. Plant tissue culture laboratories have style needs that distinguish them from alternative forms of laboratories and a few wants are distinctive for successfully plant growth as well as production of plant secondary metabolites. The most often manipulated physical parameters are light, temperature, and relative humidity within the culture vessels. The light is most important physical factor which might have an effect on metabolite production. Light not only affects the in vitro growth and developments but also very important factor affecting the production of plant secondary metabolites. The action of light on plants happens in the most part in two ways. In the first way, the light source gives the energy required by the plant for photosynthesis. In the second way, the signal which gotten by photoreceptors it regulates plant metabolism, differentiation, and growth. Temperature is different with the different in vitro life cycle, as well as the aim of production such as plant growth or secondary metabolites production.
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