Abstract
Electrochemical sensors are the tools to detect the accurate and sensitive miRs. There is the challenge to increase the power and sensitivity of the surface for the electrochemical sensor. We design a virus-like hallow structure of cuco2o4 that it holds the large amounts of p19 protein by mimicking of inherent virus (Carnation italian ringspot virus) to detect 21mir with the limit of detection (LOD = 1aM). The electrochemical measurements are performed between the potentials at −0.3 V and +0.3 V with 1 mM [Fe(CN)6] −3/−4. After dropping the cuco2o4 on the SCPE (screen carbon printed electrode), the sensor is turned on due to the high electrochemical properties. Then, p19 proteins move into the hallow structure and inhibit the exchange of electrochemical reactions between the shells and the sensor is turned off. Then, adding the duplexes of RNA/miRs cause to increase the electrochemical property of p19 due to the change of p19 conformation and the system is turned on, again. So, for the first time, a virus-like hallow structure has been used to detect the 21miR in the human serum, MCF-7, Hella cells, with high sensitivity, specificity, and reproducibility in few minutes.
Highlights
MicroRNAs are endogenous, single-stranded, non-coding small RNA with a length of ~22 nucleotide
We used as SP-300 Instruments (SP-300) Texas to detect all of electrochemical analyzes such as electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV)
In the ramnani’s research showed that the binding of p19 on the CNT/FET surface was via PBASE which it caused to reduce the resistance of the electrochemical substrate by covering the electrochemical reactions[28]
Summary
MicroRNAs are endogenous, single-stranded, non-coding small RNA with a length of ~22 nucleotide (nt). In this research; we have constructed the nanomaterials based on the inspiration of the inherent structure of CIRV to use as biosensor applications This structure consists of a mixture of metals such as cobalt and copper and oxygen (cuco2o4) which generate shells with positive and negative charges that they can create a strong bed surface. Because of the high p19 content, the sensitivity of this sensor is very high (1 aM) We use this sensor to detect 21miR in a real sample (human serum, MCF-7, Hella cells which they have the differential expression of 21 miR as model cases). This virus-like sensor can identify the miRs with high sensitivity, reproducibility, and specificity. This electrode consisted of a GNP-carbon working electrode; a carbon counter electrode and a silver reference electrode
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