Abstract
The traditional medicinal Ammi visnaga L. plants can be considered as an important economic source of raw materials for the drug industry. Nowadays, increasing the drug raw materials by using safe and favourable treatments is an important issue. So searching for the proper concentration of the suitable bioregulators which achieving high growth rate and enhancing chemical constituents of A. visnaga plants is the goal of this investigation. Foliar application of different concentrations of eitheramino acids (tyrosine and phenylalanine) and phenolic acids (trans-cinnamic acid, benzoic acid and salicylic acid) displayed significant positive effects on the accumulation of chemical constituents including phenolic compounds, flavonoids and total furanochromones in A. visnaga L. Moreover, the high-performance liquid chromatography analysis showed that some phenolic acids are highly increased, decreased or disappear in A. visnaga plants treated with various concentrations of amino acids and phenolic compounds as being compared with untreated control plants. Ferulic acid, cinnamic acid, coumaric acid and P-hydroxy benzoic acid are the most dominant phenolics in Ammi plants. Phenolics are more effective in stimulating the accumulation of phenols, flavonoids, chromones and consequently the scavenging capacity compared with the investigated aromatic amino acids. Such stimulatory effects are positively related to the applied investigated concentrations of the applied bioregulators. Indeed, Ammi plants treated with phenolics particularly benzoic acid (20 mg L−1) exhibited the greatest accumulated levels of phenolics and chromones there by enhancing thee conomic source of raw materials for drug industry.
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