Abstract
ObjectiveTo investigate the effect of G‐CSF and EPO combination therapy on functional recovery and nerve cell regeneration after traumatic brain injury in ratsMethods100 male rats were divided into Sham group, TBI group, EPO treatment group(E group ), G‐CSF treatment group(G group), and EPO combined G‐CSF group(E+G group), Neurological function was assessed using a modified neurological severity score(mNSS) before rats were sacrificed. Immunofluorescence measurement was used to detect newly proliferation cells number and its direction of differentiation by BrdU single positive cells and BrdU/NeuN or BrdU/GFAP double positive cells.ResultsThe mNSS scores of each treatment group was lower than TBI group.The BrdU positive cells in E+G group was significantly higher than other groups. Compared the groups of brain tissue BrdU/NeuN and BrdU/GFAP double positive cells number, E+G group were higher than other groups , E group and G group were higher than Sham group and TBI group.ConclusionCombined use of EPO and G‐CSF can obviously improve functional recovery and promote the brain nerve cell regeneration differentiating to neurons and astrocytes in the injury boundary zone.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call