Abstract
BackgroundAsthma is a chronic disease of the airways. Its symptoms are caused by inflammation and constriction of the bronchial muscles. During asthma there are changes in immunological pathways in which various cytokines and chemokines are involved directly or indirectly. The present study was conducted to explore the involvement of 15 Single nucleotide polymorphisms (SNPs) in 10 candidate cytokine and chemokine genes with asthma in Pakistani population.MethodsWe conducted this study in 333 asthmatic cases and 220 healthy controls.Genotyping was performed using the Sequenom Mass ARRAY iPLEX platform (10 SNPs) and TaqMan assay (5 SNPs).ResultsThe minor allele at two SNPs have shown evidence of association with risk for asthma, rs1800896 in the interleukin 10 (IL10) (OR 1.38, 95% CI 1.01–1.88, P = 0.04) and rs1800925 in the interleukin 13 (IL13) (OR 1.45, 95% CI 1.04–2.02, P = 0.03).ConclusionVariations at the IL10 and IL13 genes are found to be associated with asthma susceptibility in the Pakistani population.
Highlights
Asthma is a chronic disease of the airways
Of the 15 Single nucleotide polymorphisms (SNPs) included in our study, all were in Hardy-Weinberg equilibrium
DNA extraction, and genotyping A venous blood sample was obtained from each study participant, and genomic DNA was extracted from whole blood using a standard phenol chloroform extraction protocol [5]
Summary
Asthma is a chronic disease of the airways. Its symptoms are caused by inflammation and constriction of the bronchial muscles. During asthma there are changes in immunological pathways in which various cytokines and chemokines are involved directly or indirectly. The present study was conducted to explore the involvement of 15 Single nucleotide polymorphisms (SNPs) in 10 candidate cytokine and chemokine genes with asthma in Pakistani population. The causes of asthma are not fully understood Both genetic and environmental factors are involved, but how these factors interact to confer risk is still largely unknown. Genes involved in immunological pathways are important in asthma pathogenesis. Based on results of previous candidate gene and genome-wide studies, we selected 15 single nucleotide polymorphisms (SNPs) in 10 cytokine and chemokine genes for genotyping in Pakistani asthma cases and controls. The selection of genes is purely rely on the immunological pathways
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