Abstract
The goal of this study was to investigate potential negative sequelae of orthodontic force application ±delivery of an osteoclast inhibitor, recombinant osteoprotegerin protein (OPG-Fc), on periodontal tissues. Sprague Dawley rats from a commercial supplier were investigated in a laboratory setting. Rats were randomly divided into four groups (n=7 each): one group with no orthodontic appliances and injected once prior to the experimental period with empty polymer microspheres, one group with orthodontic appliances and injected once with empty microspheres, one group with orthodontic appliances and injected once with polymer microspheres containing 1mg/kg of OPG-Fc, and one group with orthodontic appliances and injected with non-encapsulated 5mg/kg of OPG-Fc every 3days during the experimental period. The animals were euthanized after 28days of tooth movement for histomorphometric analyses. Root resorption, PDL area and widths were similar in animals without appliances and animals with appliances plus high-dose OPG-Fc. PDL blood vessels were compressed and decreased in number in all animals that received orthodontic appliances, regardless of OPG-Fc. Hyalinization was significantly increased only in animals with orthodontic appliances plus multiple injections of 5mg/kg non-encapsulated OPG-Fc when compared to animals without appliances. Results of this study indicate that while pharmacological modulation of tooth movement through osteoclast inhibition is feasible when delivered in a locally controlled low-dose manner, high-dose levels that completely prevent tooth movement through bone may decrease local blood flow and increase the incidence of hyalinization.
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