Impact of Omega-3 on Hypercholesteremia Induced Male Rabbits

Abstract

Atherosclerosis is a disorder of vasculature wall resulting mainly from the inflammatory processes. This condition involves vascular endothelial dysfunction, recruitment and activation of phagocytic cells in early stages. Omega-3 polyunsaturated fatty acids comprise part of the cellular membrane function many roles including increased development and functionality of neuronal synapses. This study assesses the significance of omega-3 fatty acids on atherosclerosis events via lowering oxidative and inflammatory insults. In this study, twenty-four domestic rabbits (male) were randomly distributed into three groups. The first one was the negative control group in which, rabbits were received normal diet (Oxide) for twelve week-period. The second group was the hypercholesteremia-induced, untreated rabbits fed with cholesterol (1%) enriched diet. The third group consumed cholesterol (1%) enriched diet supplemented with 5% omega-3 fatty acids. Blood samples were collected after twelve weeks to measure high-density lipoprotein-C (HDL-C), total cholesterol (TC), serum triglycerides (TG), endothelin-1 (ET-1), intracellular adhesion molecule-1 (ICAM-1) and highly sensitive C-reactive protein (hs-CRP) levels. After twelve weeks, aorta of each rabbit was isolated to identify glutathione (GSH),malondialdehyde (MDA) and intimal thickness. Administration of omega-3 was un-capable to modify lipid parameters significantly when compared with high cholesterol-fed rabbits. Administration of omega-3 results in improvement of ICAM-1, hs-CRP, ET-1, aortic MDA and intimal thickness of aorta significantly when compared with untreated high cholesterol-fed rabbits (P