Abstract
Corynebacterium glutamicum is widely used for industrial production of amino acids. Recently, its ability to produce organic acids in oxygen-deprived or anaerobic conditions has also been demonstrated. In this study, the effects of oxygenation on C. glutamicum growth and organic acids production were investigated through cultures in shake flask at different kLa conditions of 0.6, 3, 11, 19, 33, 36, 48, 77, 120 and 164h−1. These values were experimentally determined in unbaffled glass shake flasks patched with oxygen probe by using the PreSens system. The results showed that increasing kLa values systematically increased growth performance. Our study also showed kLa ranges where C. glutamicum growth was altered while organic acids production was improved. In fact, the different experiments showed that (i) kLa between 10 and 19h−1 resulted in optimal succinic acid production (63mM), (ii) kLa values between 19 and 33h−1 favored high acetic acid concentrations (135mM) and (iii) kLa values higher than 164h−1 led to biomass accumulation.
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