Abstract
Corynebacterium glutamicum is widely used for industrial production of amino acids. Recently, its ability to produce organic acids in oxygen-deprived or anaerobic conditions has also been demonstrated. In this study, the effects of oxygenation on C. glutamicum growth and organic acids production were investigated through cultures in shake flask at different kLa conditions of 0.6, 3, 11, 19, 33, 36, 48, 77, 120 and 164h−1. These values were experimentally determined in unbaffled glass shake flasks patched with oxygen probe by using the PreSens system. The results showed that increasing kLa values systematically increased growth performance. Our study also showed kLa ranges where C. glutamicum growth was altered while organic acids production was improved. In fact, the different experiments showed that (i) kLa between 10 and 19h−1 resulted in optimal succinic acid production (63mM), (ii) kLa values between 19 and 33h−1 favored high acetic acid concentrations (135mM) and (iii) kLa values higher than 164h−1 led to biomass accumulation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
