Abstract
The effects of fungal pellets (FPs) dosage on both structural and functional stability of aerobic granular sludge (AGS) were investigated during 200-day operation. Results showed that the AGS induced by low (a dry mass ratio of FPs to seed sludge, 30%) and high FPs dosage (60%) exhibited good morphology integrity during the entire phase of operation, while the filamentous overgrowth and AGS breakup were observed in the control reactor (0% FPs). Moreover, the granules developed at high FPs dosage demonstrated excellent nutrients removal (COD: 93%; NH4+-N: 100%; TN: 77%) and stable bioactivity with a maximum specific oxygen uptake rate (SOUR) of 52.6 ± 2.6 mg O2/(gVSS·h), a value being 12.2% and 26.7% higher than that of 30% and 0% dosage. The microbial community analysis revealed 60% FPs dosage enriched various functional bacteria involved in nutrients removal. This study suggests a sustainable strategy for improving structural and functional stability of AGS.
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