Abstract
The ethanol extract of Mikania glomerata has anti-proliferative effect on the human breast cancer cell lines. The object of the present work is to investigate the anti-cancer effect of Mikania glomerata ethanolic extract on breast cancer. Soxlet fractions using crude ethanolic extract of Mikania glomerata was prepared by standard extraction protocols. To check the antiproliferative effect of this extract, the extract chosen was tested for cell viability on the breast cancer cells MCF 7 in different concentrations. Cell viability was evaluated by MTT assay for 24 hour and 48 hours. The LD50 value was calculated and different morphometric assays were performed with the effective dose of the extract. The effect of the extract on the normal cell was evaluated as well. Cell proliferation, cell cycle, Clonogenic survival, Apoptosis and MTT assays were performed. The ethanolic extract showed a dose-dependent and time dependent inhibition on cell proliferation in the breast cancer cell lines. It showed low cytotoxicity in the normal cells and inhibited cellular adhesion and wound healing in treated cancer cells. The present study suggests that the leaf extract from Mikania glomerata induces anticancer effect on the breast cancer cells. Further study might help to confirm it as an anti-cancer drug.
Highlights
Herbal medicine is the oldest form of healthcare known to the human race with a history of documentation for almost 4000 years
Different classes of compounds were previously isolated from various Mikania parts, which can be associated to this plant's pharmacological activities
Detailed screenings revealed the presence of other substances in species of Mikania as alcohols, acids, esters, aldehydes and organic esters [5][6]
Summary
Herbal medicine is the oldest form of healthcare known to the human race with a history of documentation for almost 4000 years. S. Sarojini et al / Impact of ethanolic extract of Mikania glomerata on human breast cancer (MCF 7) cell line on a water bath to a syrupy mass. The cell lines study was used to screen the activity of ethanolic extract of Mikania glomerata leaves against for MCF7 Human breast cancer cell line. The various treatment conditions on MCF7 breast cancer cell line with different concentration of ethanolic extract of Mikania glomerata. Aliquots (100 μL) of the culture medium containing appropriate concentrations of the test article, positive controls, and negative control were applied to the plate wells. The cells on the plate was examined microscopically to assess any morphological alterations indicative of toxicity. The assay was normally considered acceptable for the evaluation of the test results valid if the following criteria were met: No significant cytotoxicity was observed in the negative controls. The test extract is declared as a cytotoxic substance if, there was a noticeable increase (>50%) in cytotoxicity even after normalization to the negative control
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