Abstract
Influences of different extraction temperatures (50, 60, and 70°C) and time (3, 6, and 9h) on yield, physicochemical, and functional properties of gelatin from swim bladder (SBG) of rohu (Labeo rohita) were investigated. Gelatin extracted at 60°C (G60-9h), and 70°C (G70-9h) temperature for 9h showed highest gelatin yield 54.91g/100g, protein (92.35g/100g), and hydroxyproline (77.50mg/g) content (P < 0.05) compared to other counterparts. All extracted gelatin showed major protein components as α-, β-, and γ-chain with slight changes in band intensity. FTIR spectra showed significant loss of molecular order of triple-helix as temperature and time increased. Furthermore, G60-9h exhibited higher emulsion, foaming, and gel strength than G70-9h, and G50-9h, respectively (P < 0.05). Therefore, G60-9h could be appropriate conditions for the extraction of SBG with better yield, physicochemical, and functional properties.
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