Effect of various acids on physicochemical and functional characteristics of gelatin from swim bladder of rohu (Labeo rohita).

Abstract

Influence of various acids (acetic acid, phosphoric acid, and propionic acid) at different concentrations (0.05, 0.1, and 0.2M) on the extraction yield, physicochemical and functional properties of gelatin from Labeo rohita swim bladder were investigated. Highest gelatin yield (44.22%, dry weight basis) was obtained in a sample prepared by without acid pretreatment (GWA) of swim bladder as compared to acid pretreatment counterparts. Amongst the acid pretreatments, propionic acid (GPrA) showed the highest gelatin yield, followed by acetic acid (GAA) and phosphoric acid (GPA) at all concentrations used, respectively. Moreover, with increased concentrations of all acids, the decrease in gelatin yield was observed for all the acids. GWA showed higher protein and hydroxyproline content than that of acid counterparts (P<0.05). Amino acid analysis of gelatins showed glycine as the major amino acid in all gelatins followed by proline, glutamic acid and alanine, respectively. GWA showed α (α1 and α2) and β-chains as the predominant components with low molecular weight peptides. However, GPrA, GAA, and GPA had α1 and α2 dominant constituents. FTIR spectra of gelatins revealed that the loss of the triple-helix was found in GPA, GAA, and GPrA, compared to GWA. Among gelatin samples, GWA showed the highest solubility at all pH tested followed by GPrA, GPA, and GAA respectively. Furthermore, GWA exhibited higher emulsifying, foaming and gelling properties as compared to GPrA, GPA, and GAA, respectively. Therefore, the acid pretreatment of swim bladder had a negative impact on the extraction yield, physicochemical and functional properties of gelatin from rohu swim bladder.