Abstract

This study was conducted to show the effect of three different techniques of embryo freezing on the vitality and percentage of normal embryos. This research was carried out Between December 2020 and June 2021, in the Institute for Infertility Diagnosis and Assisted Reproduction at Al-Nahrain University . Ovaries were obtained from ewes that were slaughtered in a Shulla local slaughterhouse. 75 ovaries were obtained and randomly divided into three groups in this study. Oocytes were extracted from the ovaries using an aspiration procedure, followed by in vitro maturation, fertilization, early embryonic development, then vitrification, Rapid cryopreservation, slow cryopreservation, and finally vital tests and natural embryos. The nappy vitrification process was determined to be the best for cryopreservation of embryos in this investigation.

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