Decreased amount of ovarian tissue and maternal age affect embryonic development in old rats.

Abstract

The effects of addition and/or reduction of ovarian tissue and maternal age on ovulation rates (number of corpora lutea) and embryonic development were evaluated in old, regularly cycling rats on Days 4 and 11 of gestation. Young and old control rats and old rats which were either unilaterally ovariectomized (ULO), intact with 2 additional ovaries transplanted under the kidney capsule or ULO with 2 additional ovaries transplanted under the kidney capsule were mated on proestrus of a 4- or 5-day cycle between the 3rd and 9th postoperative cycle. The percentages of normal embryos on Days 4 and 11 of gestation were decreased (P less than 0.05) in the ULO rats, while on a per ovary basis the ovulation rate and ovarian weight were significantly increased in all the ULO rats compared to the old intact rats. An increase in abnormal and retarded embryos each contributed to this decreased percentage of normal Day 4 and Day 11 embryos in the ULO rats (P less than 0.05). Transplantation of ovarian tissue into old intact and ULO rats did not affect either the ovulation rate or the percentage of normal embryos and did not reverse the detrimental effects of unilateral ovariectomy. This could be due to inadequate stimulation or function of the ovarian tissue remaining in the transplants and may arise from a smaller vascular bed and limited blood flow to the transplants. Although regularly cycling young and old control rats had similar ovulation rates, the old control animals had a decreased percentage of normal embryos on Day 11 of gestation, but not on Day 4 of gestation, compared to the young control rats. This decrease in percentage of normal Day 11 embryos in the old intact rats was due mainly to an increase in retarded rather than abnormal embryos. From this study, it is concluded that unilateral ovariectomy of old cycling rats was detrimental to embryonic development. A similar, but more gradual decrease in functional ovarian tissue with aging, could cause the increased incidence of anomalies in embryos of older females.