Abstract
Treatment of aged (500day, 4°C stored) chlortetracycline (CTC; 0, 20, 40, 80mg/L CTC)-amended swine manure using two cycle, 22day stage anaerobic sequencing batch reactors (SBR) was assessed. Eighty milligrams per liter CTC treatment inhibited SBR treatment efficiencies, although total gas production was enhanced compared to the no-CTC treatment. The 20 and 40mg/L CTC treatments resulted in either slight or no differences to SBR treatment efficiencies and microbial diversities compared to the no-CTC treatment, and were generally similar to no-CTC treatments upon completion of the first 22day SBR cycle. All CTC treatments enhanced SBR gas generation, however CH4 yields were lowest for the 80mg/L CTC treatment (0.111L CH4/g tCOD) upon completion of the second SBR react cycle. After a 22day acclimation period, the 80mg/L CTC treatment inhibited methanogenesis due to acetate accumulation, and decreased microbial diversity and CH4 yield compared to the no-CTC treatment.
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