Abstract
Chlorhexidine-Digluconate (CHX-Dg) is a biocide widely used as disinfectant or antiseptic in clinical and domestic fields. It is often found in the formulation of solutions to treat superficial wounds. Nevertheless, few studies have focused on its effects on Escherichia coli while this bacterium is commonly involved in mixed infections. Therefore, the impact of CHX-Dg and temperature on E. coli was investigated; particularly the curli production. In accordance with bibliographic data, the curli production decreased when the temperature of the culture was shift from 30 °C to 37 °C. The bacterial adhesion to abiotic surfaces was also reduced. Surprisingly, the curli production at 37 °C was maintained in presence of antiseptic and the bacterial adhesion was improved at a very low concentration (1 µg ml−1) of CHX-Dg. Complementary investigations with a cpxR mutant demonstrated that the CpxA/R-TCS (Two-Component System) is involved in the temperature-dependent control of the curli expression. Indeed, the curli production was not altered by the growth temperature in the mutant. Otherwise, no relationship between CHX-Dg and the Cpx-TCS was shown. A subsequent proteomic investigation revealed the alteration of the production of 44 periplasmic and outer membrane proteins in presence of CHX-Dg. These proteins are involved in the transport of small molecules, the envelope integrity, the stress response as well as the protein folding.
Highlights
AIMS MicrobiologyCellular location Σ Coverage Σ# Peptides Σ# PSMs Score C0(1) Coverage C0(1) # Peptides C0(1) # PSM C0(1) Score C0(2) Coverage C0(2) # Peptides C0(2) # PSM C0(2) Score C0(3) Coverage C0(3) # Peptides C0(3) # PSM C0(3) Score C1(1) Coverage C1(1) # Peptides C1(1) # PSM C1(1) Score C1(2) Coverage C1(2) # Peptides C1(2) # PSM C1(2) Score C1(3) Coverage C1(3) # Peptides C1(3) # PSM C1(3) Score C2(2) Coverage C2(2) # Peptides C2(2) # PSM C2(2) Score C2(3) Coverage C2(3) # Peptides C2(3) # PSM C2(3) Score C2(4) Coverage C2(4) # Peptides C2(4) # PSM C2(4) # AAs MW [kDa] calc.
Cellular location Σ Coverage Σ# Peptides Σ# PSMs Score C0(1) Coverage C0(1) # Peptides C0(1) # PSM C0(1) Score C0(2) Coverage C0(2) # Peptides C0(2) # PSM C0(2) Score C0(3) Coverage C0(3) # Peptides C0(3) # PSM C0(3) Score C1(1) Coverage C1(1) # Peptides C1(1) # PSM C1(1) Score C1(2) Coverage C1(2) # Peptides C1(2) # PSM C1(2) Score C1(3) Coverage C1(3) # Peptides C1(3) # PSM C1(3) Score C2(2) Coverage C2(2) # Peptides C2(2) # PSM C2(2) Score C2(3) Coverage C2(3) # Peptides C2(3) # PSM C2(3) Score C2(4) Coverage C2(4) # Peptides C2(4) # PSM C2(4) # AAs MW [kDa] calc. pI Σ# Detections in C0-C1-C2
UniProt AccessionCS,OM 38 9 68 461 38 9 11 250 22 5 5 263 21 6 6 391 27 6 8 400 30 7 7 320 30 7 7 277 24 6 8 351 24 6 7 382 27 7 9 402 42 5 3-3-3.
Summary
Cellular location Σ Coverage Σ# Peptides Σ# PSMs Score C0(1) Coverage C0(1) # Peptides C0(1) # PSM C0(1) Score C0(2) Coverage C0(2) # Peptides C0(2) # PSM C0(2) Score C0(3) Coverage C0(3) # Peptides C0(3) # PSM C0(3) Score C1(1) Coverage C1(1) # Peptides C1(1) # PSM C1(1) Score C1(2) Coverage C1(2) # Peptides C1(2) # PSM C1(2) Score C1(3) Coverage C1(3) # Peptides C1(3) # PSM C1(3) Score C2(2) Coverage C2(2) # Peptides C2(2) # PSM C2(2) Score C2(3) Coverage C2(3) # Peptides C2(3) # PSM C2(3) Score C2(4) Coverage C2(4) # Peptides C2(4) # PSM C2(4) # AAs MW [kDa] calc. Cellular location Σ Coverage Σ# Peptides Σ# PSMs Score C0(1) Coverage C0(1) # Peptides C0(1) # PSM C0(1) Score C0(2) Coverage C0(2) # Peptides C0(2) # PSM C0(2) Score C0(3) Coverage C0(3) # Peptides C0(3) # PSM C0(3) Score C1(1) Coverage C1(1) # Peptides C1(1) # PSM C1(1) Score C1(2) Coverage C1(2) # Peptides C1(2) # PSM C1(2) Score C1(3) Coverage C1(3) # Peptides C1(3) # PSM C1(3) Score C2(2) Coverage C2(2) # Peptides C2(2) # PSM C2(2) Score C2(3) Coverage C2(3) # Peptides C2(3) # PSM C2(3) Score C2(4) Coverage C2(4) # Peptides C2(4) # PSM C2(4) # AAs MW [kDa] calc. pI Σ# Detections in C0-C1-C2
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