Impact and mechanism of polyethylene terephthalate microplastics with different particle sizes on sludge anaerobic digestion.

Abstract

Municipal wastewater treatment plants (WWTPs) are important sinks for microplastics, and the vast majority of microplastics entering WWTPs are trapped in residual sludge. In order to investigate the effect of microplastics on anaerobic digestion of sludge, polyethylene terephthalate (PET) microplastics with common particle size and physical aging were selected to conduct a comparative study. Regardless of aging, the addition of 300 and 500 μm PET microplastics inhibited methane production, with their cumulative methane production reduced by 11.3-24.9% compared to the control group. In contrast, when 100 μm microplastics were added, the raw PET promoted methane production, yielding 337 L CH4/kg VS, while the aged experimental group showed similar yields to the control group. For the 800 μm microplastics treatment group, aged microplastics facilitated methane production while raw microplastics inhibited it, with methane production of 91.0% and 111% of the control group, respectively. The effects were also investigated by model fitting, stage discussion, and microbial community structure analysis. The results discovered that the main rate-limiting steps of adding microplastics with smaller or larger particle sizes (100, 800 μm) to methane production were solubilization and hydrolysis, while the main rate-limiting step of microplastics with medium particle sizes (300, 500 μm) was methanogenesis. Physically aged PET microplastics with smaller or larger sizes showed a more significant effect on methane production. Furthermore, PET microplastics altered the microbial community structure, shifting methanogens from acetotrophic pathways to hydrotrophic pathways. This study offers new insights into the performance analysis of sludge anaerobic digestion in practical WWTPs.