Immunotherapy for the liver metastases prevention with a vaccine strain of an attenuated Salmonella Typhi

Abstract

Event Abstract Back to Event Immunotherapy for the liver metastases prevention with a vaccine strain of an attenuated Salmonella Typhi Alejandrina Vendrell1*, A Tesone1, J De Toro1, L Herschlik1, M J. Gravisaco2, J C. Goin1, G Larotonda3, Claudia Mongini1 and Claudia Waldner1 1 CEFYBO-CONICET/UBA, Argentina 2 Instituto de Biotecnología, CICVyA, Argentina 3 LAVET Lab Argentina, Argentina In previous studies we demonstrated that an attenuated Salmonella Typhi vaccine strain (CVD 915) induces tumor-specific immune and therapeutic responses, following intratumoral and subcutaneous inoculations, in mice bearing a subcutaneous mammary adenocarcinoma (LM3). The aim of this work was to study whether orogastric (og) administration of CVD 915 can prevent tumor cell implantation in liver, in a mouse model of liver metastases. Each BALB/c mouse received og 5x109UFC of CVD 915 or PBS (control group). After 24 hours, mice were subjected to surgery and 50,000 LM3 cells were injected into their spleens. Then, a splenectomy was performed. After 24 h of bacterial treatment, CVD 915-treated mice exhibited elevated IFN-γserum levels. Seven days later, Salmonella treatment stimulated the proinflammatory Th1 cytokines production (enhanced IFN-γ level in serum, p<0.001, and detectable serum TNF- α and IL-12 levels). The prophylactic treatment with Salmonella significantly decreased the occurrence of liver metastases as well as their average volume (1.9 vs. 6.2 mm3) 21 days after surgery, as compared with control mice (p<0.05). Moreover, at that time, an increased frequency of CD4+lymphocytes but a decreased proportion of B cells (B220+) in both liver and peripheral blood were observed in CVD 915-treated animals (p<0.05). These results indicate that a CVD 915-based immunotherapy used preventively via og route induces an antitumor immunity and prevents the implantation of metastasis in a vital organ. The use of this vaccine as an adjunct therapy to conventional cancer treatments, such as surgery, should be assessed in the future. Keywords: Cancer, Immunotherapy, liver metastasis, Salmonella typhi, mamary adenocarcinoma Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Vendrell A, Tesone A, De Toro J, Herschlik L, Gravisaco MJ, Goin JC, Larotonda G, Mongini C and Waldner C (2013). Immunotherapy for the liver metastases prevention with a vaccine strain of an attenuated Salmonella Typhi. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01202 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Sep 2013; Published Online: 11 Sep 2013. * Correspondence: Dr. Alejandrina Vendrell, CEFYBO-CONICET/UBA, Buenos Aires, Argentina, alejandrinavendrell@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Google Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Google Scholar Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner PubMed Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.