Antitumor activity of a Salmonella Typhi vaccine strain in a mouse model of T-cell lymphoma

Abstract

Event Abstract Back to Event Antitumor activity of a Salmonella Typhi vaccine strain in a mouse model of T-cell lymphoma Alejandrina Vendrell1*, A Tesone1, J De Toro1, L Herschlik1, M J. Gravisaco2, J C. Goin1, G Larotonda3, Claudia Mongini1 and Claudia Waldner1 1 CEFYBO-CONICET/UBA, Argentina 2 Instituto de Biotecnología, CICVyA, Argentina 3 Lavet Lab Argentina, Argentina Previously we demonstrated the therapeutic efficacy of an attenuated vaccine strain of SalmonellaTyphi as a cancer immunotherapy in a mouse model of subcutaneous T cell lymphoma. EL4 tumor-bearing mice treated with Salmonella Typhi CVD 915, by injection into the tumor and in the area of the draining lymph nodes, showed reduced tumor growth and increased survival. In this work, we further studied the therapeutic efficacy and the antitumor effector mechanisms promoted by this bacterium. Our results indicate that the treatment with Salmonella promoted a reduction in the mitotic index of the tumors (p <0.05) and delayed the development of palpable metastases in the lymph nodes (p <0.001). Furthermore, the administration of Salmonella in tumor-bearing mice promoted leukocyte infiltration, mainly neutrophils (p <0.01) and decreased IL- 10 levels in the tumor microenvironment (p <0.05), and also reduced LTreg frequency in tumor draining lymph nodes. Under these conditions, Salmonella remained viable within the tumor, and tumor- infiltrating neutrophils released TNF-α and expressed bacterial antigens. Finally, Salmonella was found to bind to tumor cells and exert oncolytic activity upon direct contact with the tumor cell. These results demonstrate the efficacy of a vaccine strain of Salmonella Typhi as an oncolytic and immunotherapeutic agent against a highly malignant tumor. Keywords: Cancer, Immunotherapy, T-cell lymphoma, Salmonella typhi, intratumoral therapy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Vendrell A, Tesone A, De Toro J, Herschlik L, Gravisaco MJ, Goin JC, Larotonda G, Mongini C and Waldner C (2013). Antitumor activity of a Salmonella Typhi vaccine strain in a mouse model of T-cell lymphoma. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01201 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Sep 2013; Published Online: 11 Sep 2013. * Correspondence: Dr. Alejandrina Vendrell, CEFYBO-CONICET/UBA, Buenos Aires, Argentina, alejandrinavendrell@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Google Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Google Scholar Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner PubMed Alejandrina Vendrell A Tesone J De Toro L Herschlik M J Gravisaco J C Goin G Larotonda Claudia Mongini Claudia Waldner Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.