Abstract
Summary A monoclonal antibody against a synthetic pentadecapeptide corresponding to the N-terminal end of Bombyx-prothoracicotropic hormone (PTTH: 1–15) and a monoclonal antibody against a synthetic decapeptide corresponding to the N-terminal portion of the A-chain of bombyxin-1, bombyxin (1–10), were used as parallel probes to localize both molecules at the cellular and subcellular levels in the brain-retrocerebral complex of Manduca sexta. Immunohistochemical analysis revealed that molecules immunochemically similar to Bombyx-PTTH were localized in the cytoplasm of two pairs of dorso-lateral neurosecretory cells (L-NSC III) and could be traced along their axons to the corpora cardiaca (CC) and corpora allata (CA), whereas bombyxin-like material was absent from these cells and was detected only in the cytoplasm of four pairs of median neurosecretory cells (M-NSCs). Immunocytochemical labelling at the ultrastructural level using the PTTH antibody showed high specificity with the 5 nm immunogold particles ...
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