Abstract
From a mixture of labelled proteins specific proteins can be isolated by immunological precipitation on chromatography paper disks and their radioactivity can be determined in liquid scintillation counting systems. Precipitation is completed within 2 hours. Analysis of double labelled proteins can also be performed on disks of chromatography paper. The counting efficiency for [14C]protein disks is 60%, for [3H]protein disks 8%. The statistical variation of the method (m.e.m. for n=10) is lower than 5%. Quenching, unspecific adsorption of aromatic ammoacids and immunological cross reactions are discussed. The method is especially useful when many samples are to be processed simultaneously.
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