Abstract
A simple and rapid radioassay procedure for dihydropteroate synthetase using 14C-labeled p-aminobenzoic acid as substrate has been described. The assay procedure is based on the direct measurement of the formation of 14C-labeled dihydropteroic acid from 14C-labeled p-aminobenzoic acid and 2-amino-4-hydroxy-6-pyrophosphorylmethyldihydropteridine according to prescribed conditions. The labeled dihydropteroic acid produced by the enzymic reaction is separated easily and satisfactorily from the labeled substrate by paper chromatography, and the radioactivity of the paper section containing the labeled product is counted in a liquid scintillation counting system. The formation of dihydropteroic acid can be measured at a level of 10 −11 mole by employing labeled p-aminobenzoic acid of a specific activity of 10 μc/μmole. By applying this procedure, the distribution of the enzyme in several higher plants has been investigated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
