Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha)

A.R Rocha,G.C Silva,M.M.A.S Cavalcante,R Serakides,A.M Conde Júnior,M.A.M Carvalho,N.M Argôlo Neto,M.L.T Feitosa,A.S Silva,Y.K.C Leite,D.O Bezerra,C.R.M Costa

doi:10.1590/1678-4162-10677

Abstract

ABSTRACT There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three lineages, population CD90 + and CD45 - expression, which issued high red fluorescence after the tagging with fluorescent nanocrystals by different paths and cryopreserved for future use. It is possible to conclude that mesenchymal stem cells from agouti adipose tissue have biological characteristics and in vitro behavior that demonstrate its potential for use in clinical tests.

Highlights

Mesenchymal stem cells (MSC) stand out for their plasticity
adipose-derived mesenchymal stem cells (AdMSCs) isolation started with the release of adherent fibroblastoid cells that kept its high proliferative activity and reached 80% confluence in 10 days (Figure 1)
In the culture resulting from the pellet of cells dissociated from the adipose tissue, the colonies were distributed in the culture area forming a monolayer culture

Summary

Introduction

Mesenchymal stem cells (MSC) stand out for their plasticity. The MSC were able to differentiate into mesodermal and nonmesodermal tissues, attracting even more attention in studies on their proliferation and cell specialization (Bydlowski et al, 2009). The MSC of adipose tissue were an emerging alternative since the application of autologous stem cells is prioritized in cell therapy (Nae et al, 2013). These cells promoted the recovery of several tissues and organs such as urethral smooth muscle (Zhao et al, 2012), ischemia and hepatic injury (Saidi et al, 2014), the infarcted myocardium (Gautam et al, 2015) and peripheral nerve injury (Georgiou et al, 2015)

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