Abstract
The aim of this investigation is the comparative study of changes in the surface phenotype of lymphocyte in patients with different forms of atopic diseases and latent sensitization. Materials and methods. The study was conducted on peripheral blood lymphocytes from 22 latent sensitization patients, 30 pollinosis patients, 44 atopic bronchial asthma patients and 36 atopic dermatitis patients. The control group consisted of 26 healthy people. The results of our studies demonstrate that atopic diseases are different not only in clinical manifestations, but also in mechanisms of disturbances in the functions of the immune system. Comparative study of surface markers of lymphocytes in patients with different forms of atopic diseases revealed a significant increase of surface changes of lymphocyte phenotype according to the severity of the clinical manifestations of the disease. All patients with studied forms of atopy had an increase in content of B-lymphocytes expressing CD72 marker in the peripheral blood and of lymphocytes expressing early activation antigens CD23, CD25, CD71 and adhesion receptor CD54. The developments of pollinosis, atopic bronchial asthma and atopic dermatitis are accompanied by an additional increase levels of lymphocytes expressing the late activation marker HLADR, the CD38+ precursors of plasma cells, and of lymphocytes carrying surface immunoglobulins in peripheral blood. In the blood of patients with atopic disease during exacerbation with evident clinical symptoms revealed a significant increase in all the studied populations and subpopulations of B-lymphocytes. Patients with latent sensitization had increasing blood lymphocytes expressing the CD95 receptor of Fas inducing apoptosis and low content of cells expressing its ligand CD178. Content of CD95+ lymphocytes in peripheral blood at atopic bronchial asthma and atopic dermatitis patients is reduced, and CD178+ lymphocytes increased, reflecting infringement of Fas-dependent apoptosis in severe atopic diseases.
Highlights
The results of our studies demonstrate that atopic diseases are different not only in clinical manifestations, but also in mechanisms of disturbances in the functions of the immune system
Наук, доцент, профессор кафедры патофизиологии и клинической патофизиологии, Российский национальный исследовательский медицинский университет (РНИМУ) им
Summary
Порядин Г.В., Салмаси Ж.М., Казимирский А.Н., Семенова Л.Ю. Российский национальный исследовательский медицинский университет (РНИМУ) им. Цель работы – сравнительное изучение изменений поверхностного фенотипа лимфоцитов крови у больных с различными формами атопических заболеваний и латентной сенсибилизацией. У больных всеми исследованными формами атопии отмечалось повышение содержания в периферической крови В-лимфоцитов, экспрессирующих маркер CD72, и лимфоцитов, несущих ранние активационные антигены CD23, CD25, CD71 и рецепторы адгезии CD54. У больных с атопической бронхиальной астмой и атопическим дерматитом содержание в крови CD95+ лимфоцитов, напротив, снижено, а CD178+-лимфоцитов – повышено, что отражает нарушение Fas-зависимого апоптоза при тяжелых атопических заболеваниях. Существенную роль в развитии аллергии играют нарушения активности различных субпопуляций и апоптоза лимфоцитов [1,2,3], которые могут быть исследованы с помощью оценки экспрессии отдельных функциональных молекул на поверхности лимфоцитов. Для аллергологии концепция персонализированного подхода на основе трансляционной медицины наиболее эффективно может быть реализована при изучении таких заболеваний, как поллиноз, бронхиальная астма, атопический дерматит
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