Abstract
Several herbal formulation were not properly documented due to poor scientific data as well as poor standard regulation in preparation and marketing. The study evaluate the effect of aqueous extract of Polyherbal formulation on Macrophages’ phagocytic function and Humoral immune response in Mice A total of Sixteen 16 Mice was used. Group I received normal saline, Group II-IV received 500 mg, 1000 mg, and 1500 mg of Polyherbal formulation respectively for 21 days each. The animals were sensitized and challenged with Sheep red blood cells at day 14th and 19th of the treatment respectively. On day 21st all the animals were injected with 0.1 ml Indian ink for carbon clearance assay and blood sample was collected at 1 minute and 15 minutes of the injection. The phagocytic function of Macrophages and humoral immune response were determined spectrophotometrically and Hemagglutination assay respectively. The results show that the highest carbonic particle clearances index (K) median score (Median=0.0228), Macrophage phagocytose index (α) median score (Median=3.249), organ weight index (g/100g) mean score (M=0.06633) and Hemagglutination antibody titre median score (Median=32.00) was recorded by Group IV when compared with other groups. The carbonic particle clearance (K) (p=0.02), phagocytic index of Macrophage (α) (p=0.03), and organ weight index (g/100g) (p<0.0001) significantly increase with increase in the Polyherbal formulation concentration. Hemagglutination antibody titre against Sheep red blood cells (p=0.02) also significantly increased. The extract has potential immunostimulatory activity on both Macrophages’ phagocytic function and humoral immune response in mice thus could be useful in improving immune responses.
Highlights
The knowledge of combining more than one plant material for the treatment of diseases have gain popularity in modern traditional medicine Page | 3990 (Subramani et al, 2014; Aslam et al, 2015) and preparation from this concept is referred as polyherbal formulation (PHF)
Our previous study on the PHF indicated that it has potential immunostimulatory activity in animal model (Isah et al, 2018), none has indicated its effect on Macrophage function and humoral immune response against sheep red blood cells (SRBCs)
Humoral immune response constitute a component of adaptive immune response and is responsible for synthesis of antibodies secreted by plasma cells which develop from B lymphocytes (Fuza and Skok, 2007, Hajra et al, 2012)
Summary
The knowledge of combining more than one plant material for the treatment of diseases have gain popularity in modern traditional medicine Page | 3990 (Subramani et al, 2014; Aslam et al, 2015) and preparation from this concept is referred as polyherbal formulation (PHF). Several herbal formulation were not properly documented due to poor scientific data as well as poor standard regulation in preparation and marketing (Kazeem et al, 2013). This may risk the life of individuals due to adverse toxicity (Oshikoya et al, 2008). Our previous study on the PHF indicated that it has potential immunostimulatory activity in animal model (Isah et al, 2018), none has indicated its effect on Macrophage function and humoral immune response against sheep red blood cells (SRBCs). This study will offer scientific information on the immunomodulatory potential of aqueous PHF extract in mice targeting Macrophage phagocytic function and the humoral immune response against SRBCs. In addition, some medicinal plants have an effect on the phagocytic activity of Macrophages (Ranjith et al, 2015). Humoral immune response constitute a component of adaptive immune response and is responsible for synthesis of antibodies secreted by plasma cells which develop from B lymphocytes (Fuza and Skok, 2007, Hajra et al, 2012)
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