Immunomodulatory action of jacalin from Artocarpus integrifolia and mannoprotein from Saccharomyces uvarum on the humoral immunity of laying hens

Marco Aurélio Chiara Silva,Alice Eiko Murakami,Emerson José Venancio,Wagner Loyola,Miriele Caroline Da Silva,João Waine Pinheiro,Raul Jorge Hernan Castro-Goméz

doi:10.1590/0103-8478cr20190700

Abstract

ABSTRACT: Advances in the fields of glycobiology and immunology have provided many insights into the role of carbohydrate-protein interactions in the immune system. Jacalin of Artocarpus integrifolia (JCA) and structural mannoprotein of Saccharomyces uvarum (MPS) are molecules with immunomodulatory properties. JCA is an IgA human lectin binding molecule that causes the mitogenic stimulation of immune cells, production of cytokines, chemotaxis, and activation of leukocytes. Studies on the immunomodulatory properties of JCA and MPS in mammals and fish suggest that they have an action on antibody production. The aim of this study was to investigate the possible action of JCA and MPS on the production of specific antibodies in laying hens. For this, laying hens were inoculated with an intra abdominal injection of sheep red blood cells (SRBC) with either JCA (0.075 µg, 0.75 µg, and 7.5 µg) or MPS (20 µg and 100 µg). Levels of anti-SRBC antibodies of the IgY, IgM, and IgA classes were evaluated by ELISA. Results showed that JCA and MPS have immunomodulatory effects on levels of anti-SRBC IgM, IgA, and IgY. An immunostimulatory effect of JCA was observed in primary immune response on anti-SRBC IgY, while an inhibitory effect of JCA and MPS was observed in secondary immune response on the production of IgM and IgA anti-SRBC. These results suggested that MPS and JCA have immunomodulatory effects on antibody production and could be used in future studies on humoral immune response in poultry.

Highlights

Immunomodulators are molecules that exert action on the immune system, which may result in immune system activation or suppression (MASIHI & SCHÄFER, 2011)
It was shown that the ethanol extract of propolis has an adjuvant effect in laying hens and can be used for the development of novel adjuvants (FREITAS et al, 2011), while sulfated polysaccharides and carboxymethyl glucan have an adjuvant effect on the humoral and cellular immune response in chickens vaccinated against Newcastle disease and avian influenza (GUO et al, 2012; DARPOSSOLO et al, 2010)
Several studies have demonstrated that JAC is important as a lectin with diverse applications, ranging from the isolation of human IgA1 to applications in acquired immune deficiency syndrome (AIDS) research (KABIR, 1998; MADRUGA et al, 2014)

Summary

Introduction

Immunomodulators are molecules that exert action on the immune system, which may result in immune system activation or suppression (MASIHI & SCHÄFER, 2011). Many immunomodulatory molecules are essential components of adjuvants used in vaccines and are directly related to humoral immune response stimulation and the production of antibodies (JIN al., 2018, NICHOLLS et al, 2010). The main adjuvants used for the production of immunoglobulin IgY antibodies in laying hens are complete (CFA) and incomplete (IFA) Freund’s adjuvants. The identification of immunomodulatory molecules would be a useful tool to study humoral immune response and IgY production in chickens. It was shown that the ethanol extract of propolis has an adjuvant effect in laying hens and can be used for the development of novel adjuvants (FREITAS et al, 2011), while sulfated polysaccharides and carboxymethyl glucan have an adjuvant effect on the humoral and cellular immune response in chickens vaccinated against Newcastle disease and avian influenza (GUO et al, 2012; DARPOSSOLO et al, 2010)

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