Abstract
Seventy-nine children participated in the study including 33 children living in region with developed cement industry (12 with glomerulonephritis, and 21 with obstructive pyelonephritis). A group of comparison consisted of 46 children living in Moscow, including 17 subjects with glomerulonephritis and 29 children with obstructive pyelonephritis, and control group of 26 healthy children. ELISA method was used to perform immunological studies. The levels of sCD4, IL-2, IL-6, IL-10, sICAM-1, TNFα were evaluated in blood sera. The data obtained show significant increase of TNFα levels and decreased IL-6 levels (p < 0,05) in children with nephropathy living in regions with unfavorable environmental conditions as compared with control group. A seven-fold increase in TNFα levels, along with more than twofold decrease in IL-6 was revealed among children with obstructive pyelonephritis, as compared with control group (p < 0,05). In children with glomerulonephritis and obstructive pyelonephritis, a distinct increase of sCD4 , as well as decreased IL-2 level (p < 0,05) was registered, as compared with control group. Meanwhile, IL-10 contents in this group of patients was 22,4 times less than the in controls (p < 0,05). In the main group, no enhanced sICAM synthesis was found, both in children with glomerulonephritis and pyelonephritis. In children affected by adverse environmental pathogens, we have shown a prevailing immune inflammation due to hyperproduction of TNFα, IL-6, and activation of sCD4 helpers. Adverse environmental effects inhibit cytokine synthesis, thus reducing production of both pro- and anti-inflammatory cytokines.
Highlights
Seventy-nine children participated in the study including 33 children living in region with developed cement industry (12 with glomerulonephritis, and 21 with obstructive pyelonephritis)
The data obtained show significant increase of TNFα levels and decreased IL-6 levels (p < 0,05) in children with nephropathy living in regions with unfavorable
In children affected by adverse environmental pathogens, we have shown a prevailing immune inflammation due to hyperproduction of TNFα, IL-6, and activation of sCD4 helpers
Summary
Иммунологическое исследование у детей с заболеваниями почек, проживающих в регионах с экологическим неблагополучием. Иммунологические исследования по содержанию в сыворотке крови sCD4, IL-2, IL-6, IL-10, sICAM-1, TNFα проведены с использованием метода ИФА. У детей с нефропатиями в экологически неблагоприятных регионах отмечено значительное по сравнению с контрольной группой увеличение уровня TNFα и снижение уровня IL-6 (p < 0,05). При обструктивном пиелонефрите выявлено увеличение уровня TNFα у детей контрольной группы в 7 раз по сравнению с основной группой (p < 0,05); уровень IL-6 был в 2 и более раз ниже. При гломерулонефрите и обструктивном пиелонефрите был значительно повышен sCD4 и снижена концентрация IL-2 (p < 0,05); содержание IL-10 было в 22,4 раза меньше по сравнению с контрольной группой (p < 0,05). В основной группе не зарегистрировано увеличение синтеза sICAM-1, как при гломерулонефрите, так и при пиелонефрите. В условиях воздействия экопатогенов на организм ребенка доказано преобладание иммунного воспаления за счет гиперпродукции TNFα, IL-6 и активации хелперов sCD4.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
