Abstract
This chapter presents the immunological studies on membrane associated antigens from renal adenocarcinoma and human kidney cortex using polyclonal and monoclonal antibodies. Isolated plasma-membranes (PM) from renal adenocarcinoma exhibited not the asymmetric fine structure found on PM from human kidney cortex, due to the fact that tumor PM lack major surface lectin receptors present on kidney PM. This observation was supported by quantitative histophotometry of tumor slices using image analysis device. However, ϒ-Glu-transpeptidase, a constant marker of PM from renal carcinoma, revealed a significantly higher affinity towards immobilized Con A compared to ϒ-Glu-transpeptidase from kidney PM. Rabbit antisera directed against PM of renal adenocarcinoma gave only a weak immuno-fluorescent staining of tumor tissue. Production of monoclonal antibodies against isolated PM of human kidney and renal carcinoma resulted in a great variety of antibodies with high specificity against nearly all known, histologically defined structures of the nephron. These findings support the idea of presence of cryptic antigens or synthesis of neoantigens on the tumor membranes.
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