Immunological function analysis of school-age children with henoch-schonlein purpura in acute phase

Abstract

Objective To investigate the immunological function of school-age children with Henoch-Schonlein purpura(HSP) in school-age children in acute phase,in order to investigate the role of immunological injury in pathogenesis of HSP.Methods Eighty children with HSP in acute phase were enrolled as research gruop in this study,witch were divided into purpura nephritis (HSPN) group and nonnephritis (NHSPN) group according to absence of renal injury; And 20 healthy children were enrolled as control group.The antigen markers of T-lymphocyte,B-lymphocyte and NK cells,cluding CD4,CD3,CD8,CD4/CD8,CD16/56 and CD19 expression on the surface of peripheral blood lymphocyte were analyzed by flow cytometry,the serum levels of IgG,IgA,IgM,IgE and the C3,C4 were detected by immunol nephelometer.Results IgA,IgE,the rato of IgA/C3,CD19 + cell in HSP group were higher than those in control group ; CD3 +,CD3 + CD4 +,the ratio of CD4 +/CD8 + cell were lower than those in control group.The ratio of IgA/C3 in HSPN group was higher than that in NHSPN group; The ratio of CD4+/CD8 + in HSPN group was lower than that in NHSPN group.Conclusions There are abnormal changes in humoral immunity and cell mediated immunity in acute phase of HSP,and the ratio of CD4 +/CD8 + and IgA/C3 may be the assessment indexes to predict whether it is prone to have renal injury. Key words: Henoch schonlein purpura; Purpura nephritis; Children; Humoral immunity; Cell mediated immunity