Abstract
Purpose: To investigate the function of human corneal endothelial cells (HCEC) as immunological cells. Methods: Expression of HLA-DP, -DQ, -DR, CD40, CD80 and CD86 was determined by immunohistochemical methods. Purified peripheral blood mononuclear cells were cocultured with HCEC, part of which had been pretreated with γ-interferon (IFN), and activation of lymphocytes was determined by fluorescence-activated cell sorting analysis. Results: In the coculture system, T lymphocytes were activated by corneal endothelial cells; HLA-DP, -DQ, -DR and CD40 expressions were increased by γ-IFN pretreatment. Costimulatory CD80 was shown on the endothelial cells. Conclusions: HCEC can be assumed to be involved in the rejection process of corneal transplants as potential antigen-presenting cells.
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