Abstract
Antigens were isolated from moulds ( Penicillium verrucosum var. cyclopium, Mucor racemosus and Fusarium oxysporum) by a simple water extraction followed by column chromatography using Sepharose CL4B. The antigens obtained were genus-specific, heat-stable and found not to be present in extracts of non-moulded food. Immunoglobulins were produced against these antigens and an enzyme-linked immunosorbent assay (ELISA) was developed. Using the ELISA, moulds were detected in both heated and unheated foods in a sensitive and specific way. The results indicate the possibility of testing whether prepared foods are produced from non-moulded raw materials or not. Heat treatment during processing does not influence the recovery of mould contamination.
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