Abstract
sputum is a non-invasive technique used to collect cells and soluble material from the central airways. The method is feasible to assess airway inflammation among workers exposed to different organic and inorganic particles and gases. In the present review we discuss different immunological methods that are used to analyze induced sputum with a focus on occupational exposure. These methods include differential cell count, flow cytometry, analysis of protein, gene expression, DN a and particles as well as in situ hybridization and immunocytochemistry. We discuss changes in induced sputum after non-specific and specific exposure and possible methodological pitfalls.
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