Abstract
Polyclonal antibodies were raised against proteinase K and were used to immunolocalize the major extracellular proteinase of the sap-staining fungus Ophiostoma piceae (Münch) H. and P. Sydow. Immunodot blotting showed that the IgG antibodies recognized both enzymes but reacted more strongly with proteinase K than with the O. piceae proteinase. Immunogold labelling and transmission electron microscopy revealed that the O. piceae proteinase was localized in the cell walls of O. piceae grown either in liquid media or wood. Key words: Ophiostoma piceae, proteinase, immunogold labelling, transmission electron microscopy, antibody, proteinase K.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
