Abstract
Abstract The specificity of nine monoclonal anti-CEA antibodies was studied using immuno-histology and immuno-chemistry. The anti-CEA antibodies were tested on normal lung, liver, stomach and spleen, which are known to contain the cross-reacting antigens NCA, NCA2 and BGP1 and on normal colon and colon carcinoma which contain CEA. The results showed that in immuno-blotting all monoclonal anti-CEA antibodies reacted with an antigen with a molecular weight of 190,000, extracted from coloncarcinoma. Immuno-histology demonstrated that 8 monoclonal anti-CEA antibodies were directed against epitopes present on both CEA as well as on cross reacting antigens present in normal tissue. One monoclonal antibody, however, stained only normal colon and colonic carcinoma. These results demonstrate that monoclonal anti-CEA antibodies can be produced which are able to bind to a specific CEA epitope. Because the epitope recognized is present in the majority of epithelial malignancies and only in small amounts in normal colon mucosa, this anti-CEA antibody seems very suitable for use in immunoscintigraphy and possibly in immunotherapy.
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